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Finally, report with rigor so decisions are reproducible and mechanism-anchored: adhere to Methods for ROS detection: techniques, limitations and pitfalls section standardsinclude specificity controls (SOD/catalase, chelators), calibrations and kinetic context and compartment targetingso that therapeutic readouts map cleanly to species, source and location, allowing precise titration rather than blanket scavenging or untargeted escalation

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In the same regard, mushroom cultivation at the industrial level produces huge amounts of a byproduct called spent mushroom substrate comprises a mixture of remaining substrate and mushroom mycelia [222]

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