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Quantitative PCR After the determination of the antibiotic resistance genes of identified Escherichia coli and Pseudomonades aeruginosa , quantitative real-time PCR (qRT-PCR) was utilized for quantification of the resistance genes in the identified bacteria

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Therefore, in the context of inflammatory syndromes, despite high vitamin B12 blood levels, there is a vitamin B12 deficiency in target tissues, potentially leading to hyperhomocysteinemia and increased cardiovascular risk [69]

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