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One or more interactions may be part of the proposed antioxidant mechanism of CCM, like neutralizing or scavenging free radicals, preventing oxidative cascades, quenching oxygen, inhibiting oxidative enzymes, and deactivating toxicants oxidative properties (Rao 1994

People of thiamin deficiency include those affected by: pregnancy chronic illness inadequate dietary intake alcoholism bariatric surgery chronic diuretic therapy Prolonged, untreated thiamin deficiency may lead to Wernicke-Korsakoff syndrome, a disorder that can affect people with chronic alcoholism, gastrointestinal disorders, or AIDS

To introduce single-site mtDNA mutations ( TrnE G14702A mutation, ND5 G12918A mutation) in 4T1 cells in vitro , 2.5 g of each DdCBE plasmid pair were transfected into 6*10 5 cells using the Lipofectamine 3000 Transfection Reagent (Invitrogen)