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Cancer Target Therapies Oncol

The mechanism of action is closely linked to the multitargeted properties of natural products: Rikkunshito stimulates appetite by promoting ghrelin secretion and creates a more recoveryfriendly physiological environment by regulating gastrointestinal motility and hormone levels [60]

For protein purification, harvested cells were resuspended with ice-cold lysis buffer (50 mM Tris-Cl pH 8.0, 300 mM NaCl, and cOmplete, EDTA-free protease inhibitor cocktail tablets (Roche)) at a ratio of 3.2 mL/g of cell pellet

HepG2-based reporter cells were generated by Bacterial Artificial Chromosome tagging as described previously (Wink et al., 2017)