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2.5 Determination of protein concentration The concentration of purified proteins was determined using Bradford reagent (Sigma-Aldrich) according to the manufacturers instructions with bovine serum albumin as a standard

GO enrichment analysis revealed that the key BP terms were primarily associated with response to xenobiotic stimuli, response to oxidative stress, response to nutrient levels, and the regulation of chemical synaptic transmission

Additionally, developing novel efficacy endpoints beyond conventional CAP, such as evaluating the specific modulation of hepatic triglyceride compositionparticularly the proportion of lipotoxic saturated fatty acidsmay more directly elucidate the mechanisms underlying its hepatoprotective effects

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