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Monocyte isolation and cell culture Human peripheral blood mononuclear cells (PBMCs) were isolated from the obtained leukopaks using density gradient centrifugation with Ficoll-Paque Plus (Cytiva, Marlborough, MA)

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As a component of complex enzymatic systems, cobalamins participate in many key endogenous transformations, including, among others, the synthesis of nucleic acids, membrane phospholipids, neurotransmitters, and the proper functioning of the myelin sheaths of nerve fibers