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18 NOX Complexes: Subunits and Regulation Mechanisms Although they share a very similar catalytic core architecture, the subunits that constitute the multiprotein complex of individual NOX proteins are different

doi: 10.5455/javar.2020.g414 83 McCollumM

Forward primers for mutagenesis were: R38A: CTC CGC GAC TGC ATT CCA GGC GCT AAC CAA CAA GGT TTT TG R130A: CCC TTC CTC AGC CAA ACG CGG GCT TGG CAT TTT TTG Mutants were expressed and activity measured in membrane fractions as described 47

Next, we compared the frequency of Ent1 or Ent2 at endocytic sites which are Abp + , between WT and Cla+Arr double mutant cells