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This can be easily explained by the fact that NAC is often used concurrently with pro-oxidant agents and can reduce oxidative stress, most probably by reacting directly with them in the cell culture medium, and even when a pretreatment is declared, in most cases NAC is not washed away before the oxidative insult

doi:10.1074/jbc.M805586200, PMID: 18990698 Borutaite V, Brown GC (2007) Mitochondrial regulation of caspase activation by cytochrome oxidase and tetramethylphenylenediamine via cytosolic cytochrome c redox state

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Rognoni A, Cavallino C, Veia A, et al