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Description
Stable transfection of the human parasite Leishmania major delineates a 30-kb region sufficient for extrachromosomal replication and expression
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Enzyme assay and kinetic assay Screening assays and kinetic assays were conducted in 500 L volumes in a micro-cuvette (104.002B-QS 10 mm), and absorbance was measured using a spectrophotometer at 274 nm and 25 C, and compared to the absorbance of no-enzyme controls ( n = 3 for each treatment)

GRANTS This work was supported by National Heart, Lung, and Blood Institute Grants P01-HL-074237, HL-105301, and T32-HL-072751
