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2.4.1 Ultrafiltration The first step in the purification process of antioxidant collagen hydrolysates is usually ultrafiltration membrane separation, due to its advantage of simple operation, low energy consumption and environmental friendliness [63]

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The MultiCloning Site (MCS) of a pET-52b(+) vector (Novagen), containing a 10xHis-tag, was inserted into the MCS of a pFastBac1 vector to generate pFastBac1-Strep-10xHis
