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NAC also suppressed elastase release from neutrophils induced by formyl-methionyl-leucyl-phenylalanine (fMLP) in a dose dependent manner, as the elastase was completely abolished by 10mM NAC (Figure 3)

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Eluent B was prepared by adding 15 mL of 500 mM 1-octanesulfonic acid sodium salt solution (Nacalai Tesque) and 0.2 mL of trifluoroacetic acid (Nacalai Tesque) to 300 mL of acetonitrile for HPLC (Nacalai Tesque), with a total volume of 1000 mL made up with distilled water for HPLC (Nacalai Tesque)

REFERENCES 1 AnagnostisP.AthyrosV