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The data, together with the data from the same study measuring pyruvate levels described above, indicate that fasting increased the fumarate/pyruvate, and so also likely increased the malate/pyruvate, indicative of the [NADPH]/[NADP + ], in the spinal cord and several brain regions, including the hippocampus, brainstem, olfactory bulb, and potentially to a smaller extent in the cortex, while potentially decreasing in the hypothalamus (Shao et al., 2023)

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Antibodies used in Western blotting were: anti-METTL3 (Abcam, ab195352, clone [EPR18810], 1:1000), anti-FTO (Abcam, ab126605, clone [EPR6894], 1:5000), anti-YTHDF1 (Proteintech, 174791-AP, 1:1000), anti--actin (Sigma-Aldrich, A5441-.2 ML, clone AC-15), anti-CHIKV capsid rabbit polyclonal antibody (1:5000), a gift from Prof