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Actinomycin D (AMD) (A1410) and rapamycin (553210) were purchased from Sigma
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Preparation of water soluble lens proteins Four de-capsulated lenses from each group were homogenized in 600 L of lysis buffer (20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na 2 EDTA, 1 mM EGTA, 1% Triton, 2.5 mM sodium pyrophosphate, 1 mM -glycerophosphate, 1 mM Na 3 VO 4 , 1 g/mL leupeptin, and 2 mM PMSF
Clinical phenotype and genetic associations in autosomal dominant familial Alzheimers disease: a case series
