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Before the experiments, the worms were rinsed off the growth plates and washed three times in CTM (5 mM potassium phosphate, pH 6.0, 1 mM CaCl 2 , 1 mM MgSO 4 ) to remove bacteria
At the end of the permeability assay midbrain organoids were removed from the bottom of acceptor compartments, homogenized in DW containing Triton X-100 (10 mg/ml)
The human GPX3 gene consists of five exons in the 5q32 region of chromosome 5, it is 10 kb in length and it encodes a 23-kDa protein that forms a homotetramer (14)
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