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The use cannot be discontinued (use ceases for 12 consecutive months or ceases to be licensed by the California Department of Alcoholic Beverage Control ABC) 3

This cholesterol-dependent -barrel cytolysin binds to target cells, assembles into the membrane, and forms pores (122)

The size of the molecules selected may permit understanding of the different routes of transport, with molecules such as inulin, polyethylene glycols (PEGs) and Fluorescein Isothiocyanate-Dextran (FITC-Dextran) being utilised for assessment of the paracellular route, whilst larger molecules such as horseradish peroxidase can be utilised for assessment of the transcellular route [21]

doi: 10.1016/j.cmet.2018.02.024 284 YangWBaiYXiongYZhangJChenSZhengXet al