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coli NEBExpress was first transformed with the pKD46 plasmid and then typically grown in 2 L Terrific Broth (TB) medium supplemented with 100 g/mL Amp at 30 C in the presence of 0.2 % (w/v) arabinose to induce expression of the Red genes

Katz LE, Ferry RJ, Stanley CA, Collett-Solberg PF, Baker L, Cohen P: Suppression of insulin oversecretion by subcutaneous recombinant human insulin-like growth factor I in children with congenital hyperinsulinism due to defective beta-cell sulfonylurea receptor

Monti M, Agamennone M, Wong MCY, Calevo MG, Losurdo G, Avanzini S, Mattioli G

Archives of Virology, Volume 170, article number 1