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24 h later, culture media were replaced with 1 mL of serum-free DMEM containing 50 ng/mL of recombinant hTNF- (Peprotech) and cultured for a further 24 h before collecting cells and supernatants
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Miotto G, Rossetto M, Di Paolo ML, Orian L, Venerando R, Roveri A, et al
Trained immunity induction by the inactivated mucosal vaccine MV130 protects against experimental viral respiratory infections
