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For genetic complementation, a DNA fragment containing 500 bp upstream of MSMEG_5827 (including its native promoter) was amplified and cloned into pHY plasmid via Gibson assembly to generate pHY- MSMEG_5827

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The satellite cell activation is particularly importantthese muscle stem cells normally remain quiescent but can be recruited by IGF-1 signaling to fuse with existing fibers, adding nuclei that support increased fiber size

28 GadjevI.VanderauweraS.GechevT