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Ethics declarations Ethics approval and consent to participate The study protocol was conducted according to the guidelines contained within the Declaration of Helsinki and was approved by the ethics committees of Yokohama City University and Kyoto Prefectural University

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The availability of chemically defined NAPQI enabled these studies to establish that differences in species sensitivity were due to differences in the rate of metabolic conversion of APAP to NAPQI.14 The aforementioned in vitro studies were performed using hepatocytes from a relatively small number of donors ( n = 411)