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While vitamin B 12 deficiency is not a primary test in newborn screening programs, a study showed that markers C3, C3/C2, and C3/C16 exhibit a negative correlation with maternal levels of vitamin B 12 in the first trimester of pregnancy, and thus may bear diagnostic value (Dayaldasani et al., 12 insufficiency or deficiency during the first trimester of pregnancy and adequate treatment

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Vitamin B12 deficiency may cause tiredness, loss of appetite, constipation, weakness, weight loss, neuropathic problems and megaloblastic anaemia