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Persistent synovitis can induce VEGF production, promoting neovascularization and increased vascular permeability (143), which may facilitate the influx of iron into the joint cavity and subchondral bone and recruit additional immune cells, thereby intensifying local oxidative stress and inflammatory cascades (144)

Higher concentrations are achieved by using mixtures (for example 15% DMSO in combination with 15% ethylene glycol) which reduces the amount and toxicity of the individual cryoprotectants 10

10.1104/pp.106.089458 64 NegiY

(2014) 'Induction of genomic DNA hypomethylation in the offspring of rats consuming diets mildly deficient in vitamin B2, B6, B12 and folate', *Nutrition*, 30(8), pp