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Several studies have shown that HMOX2 gene expression is activated by oxidative stress, while hypoxia can regulate gene expression and translation (Muoz-Snchez and Chnez-Crdenas, 2014 for a review)
doi: 10.1016/j.pnpbp.2011.08.018 216
The subsequent steps followed the same protocol as described above for whole-mount immunofluorescence staining
However, this decline was estimated based on a conservative Greenland shark life span of 150 years
