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Each course should be followed by a pause of at least four months

Regulation of human alpha-globin gene expression and alpha-thalassemia

The samples were then desalted using a modified version of Rappsilbers protocol 88 in which the dried samples were reconstituted in acetonitrile/water/TFA (solvent A, 100 L, 2/98/0.1, v/v/v) and then loaded onto a small portion of a C18-silica disk (3 M, USA) placed in a 200 L pipette tip

Koubaa A, Triki M, Trabelsi H, et al