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A., Despommier, D
Int J Stroke 13:612632
The reaction time was 48 h for first and second reactions, 168 h and 216 h for the third and fourth reactions and the fifth reaction, respectively
Thirty or forty micrograms of proteins were loaded into each well and fractionated on 510% precast SDS-PAGE gels (Byeotime, Shanghai, China) and then transferred to a PVDF membrane (Merck Millipore, Darmstadt, Germany)
