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Individual fiber bundles were then separated with two pairs of sharp forceps and then the fiber bundles were permeabilized for 30 min in 3 ml of ice-cold isolation solution with saponin (50 g/ml)

Arch Microbiol 162, 243248

Spherocytes are found in all hemolytic anemias to some degree
Traditional Chinese medicine in the treatment of chronic kidney diseases: theories, applications, and mechanisms