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After an additional incubation for 2 h at 37 C, absorbance was measured at 450 nm using an ELISA Microplate Reader (VersaMax, Molecular Devices, USA)
Galectin-1 specifically modulates TCR signals to enhance TCR apoptosis but inhibit IL-2 production and proliferation

Follow the product instructions carefully, as the recommended dosage typically decreases during maintenance

coli His-GST-lambda protein phosphatase (made in house) to DCAF16 protein at 4C overnight in 20 mM HEPES, 150 mM NaCl, 1 mM TCEP pH 7.5 buffer supplemented with 1 mM MnCl 2