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After 24 hours from the first trial, a second trial is performed by placing the animal in the same container, but for 5 minutes

Selective and membrane-permeable small molecule inhibitors of nicotinamide N-methyltransferase reverse diet-induced obesity in mice. Nature Medicine, 25(11), 17611771

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Methods CAD patients were identified by cardiac catheterization as having at least 50 % stenosis of one major coronary artery
