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Description
Mutant IDH1 leads to the accumulation of the metabolite 2-hydroxyglutarate (2-HG), which stabilizes HIF-1, further promoting immune suppression in the TME
Its fine for immediate, one-shot dilutions but unsuitable for ongoing research protocols
In brief, cells were dissociated, resuspended, and incubated with 200 nM MitoSpy Green FM in complete medium without serum at 37 C for 10 min
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