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Through injection of -syn preformed fibrils (PFFs) into the striatum of transgenic mice, researchers demonstrated the development of Lewy pathology, nigrostriatal degeneration, and importantly expanded our understanding of cell-to-cell transmission by describing the nature of spread in neuroanatomically connected regions: this provided the first evidence that synthetic -syn PFFs alone can induce the initiation and propagation of -syn pathology in vivo (Luk et al., The evidence of cell-to-cell transmission of pathologic -syn in interconnected brain regions suggests a prion-like mechanism of spread

doi: 10.1016/j.fertnstert.2017.01.001 5

After rinsing with PBS, the sections were incubated with Cy3-conjugated goat-anti-mouse (115-165-003, 1:200, Jackson Immuno Research) for 1 hour at room temperature in the dark

Regardless of the cause of CP, immune cells play an important role in the pathogenesis and severity of the disease