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Description
This cellular HTS is based on protein-fragment complementation assays (PCAs), in which the protein of interest is fused to fragments of a reporter protein whose activity is recovered upon binding

The mechanism is proposed to involve G-protein coupled receptor signaling, but definitive identification of the functional receptor has not been published

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The methods involve the reduction of Cu(II) in its complexes with neocuproine (NCN), bicinchoninic acid (BCA), and bathocuproine disulfonic acid (BCS) to the corresponding chromophoric Cu(I) complexes by the analyte
