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**tartrazine: may have an adverse effect on activity and attention in children
The relative abundances of Clostridium subcluster 4 of bacteria in the fecal samples were evaluated using quantitative PCR, which was performed using SYBR Green (Takara Bio, Kusatsu, Shiga, Japan) and an Mx3000P real-time PCR system (Agilent Technologies Japan Ltd., Tokyo, Japan), as reported previously [9]
Pt 1Int
