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Description
To address heat-induced changes in metabolic activity, 1.5-ml microcentrifuge tubes with 500 L3 in 500 l HBSS-AB were placed in a water bath and incubated for varying times (1 to 8 min) at 60 C and allowed to stand at RT for 20 min before transferring L3 into a 96-well plate with a final volume of 100 l/well in triplicate
Hawley S
No one media is universally used, though a move away from serum-based media and towards DMEM or neurobasal media was seen following the finding that serum-based media can lead to significant drift from the parent tumor 21
Km 40mg: Tng cng h min dch, h tr lnh vt thng
