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Free cholesterol is acquired from macrophages and other peripheral cells and esterified by LCAT, forming mature HDLs
Gut Flora Metabolism of Phosphatidylcholine Promotes Cardiovascular Disease. Nature 472: 5763

Garcia GA, Nguyen CV, Vo TA, Yonkers MA, Minckler DS, Tao JP

The addition of chloroform should generate two separate phases
