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7.2.3 Other factors that may affect the efficiency of dsRNA processing and siRNA function For an alphanodavirus that persistently infects Hi5 cells, a lepidopteran cell line derived from Trichoplusia ni , a phased pattern of vsiRNAs with periodicity of 20 nt was observed, indicating that Dcr-2 in Lepidoptera is a processive enzyme like in Drosophila , capable of translocating along the dsRNA substrate in an ATP-dependent manner (224)