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Culture-Independent Approach Besides that, different approaches have been developed to study the microbiome, including clone libraries, terminal restriction fragment length polymorphism (T-RFLP), quantitative PCR (qPCR), transcriptome microarrays, as well as high throughput sequencing technologies (Illumina sequencing and 454 pyrosequencing)

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Thus, we propose that the primary objective of filament formation in CBS is to increase the kinetic stability of the regulatory domain to maintain the basal conformation of the enzyme 28,29,35 (Fig

in other words, every compound showed at least one parameter predicted as active or inactive