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The fluorescence intensity of SF650T-labeled Halo7-Rac1 released from the EVs in the endosomes into the cytoplasm in the recipient cells was measured as follows: puncta of the Halo7-Rac1, as Halo7-Rac1 remained in the EVs in the endosomes, were detected using Analyze Particles plugin of Image J as the particles with a threshold of more than 3000

10.1007/s11882-010-0154-7 17 MuraroA.WormM.AlvianiC.CardonaV.DunnGalvinA.GarveyL