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Lentiviral vector production and cell transduction Transfection mixture was prepared in 800 L OptiMEM (Gibco) by adding following plasmids: 12 g of psPAX2 (AddGene #12260), 5 g of pVSV-G (AddGene #138479) and 12 g of expression vector carrying shRNA sequence, in the presence of 80 L of TransIT- LT1 Transfection Reagent (Mirus, Mir2360)

[DOI] [PubMed] [Google Scholar] 10.Akgun S, Ertel N, Imperato-McGinley J, Sayli B, Shackleton CHL

PMID: 28235209

Visual Asset: Alcohol Absorption Pathway Diagram Recommended: Simple anatomical illustration showing the GI tract with two labels Stomach (20% absorption) and Small Intestine (80% absorption) with an arrow showing the pyloric valve between them