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Peptides were separated on an Ultimate 3000 RSLC nano-HPLC system using a pre-column for sample loading (Acclaim PepMap C18, 2 cm 0.1 mm, 5 m), and a C18 analytical column (Acclaim PepMap C18, 50 cm 0.75 mm, 2 m, all HPLC parts Thermo Fisher Scientific), applying a linear gradient from 2 to 35% solvent B (80% acetonitrile, 0.1% formic acid
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